The Live Nagpur
  • Nagpur City
  • Nagpur Education
  • Nagpur Sports
  • Business
  • Crime
  • Entertainment & Lifestyle
  • Featured
  • Health & Wellness
  • Nation
  • Politics
  • Trending
  • Vidarbha
  • Obituary

The Live Nagpur

  • Home
  • Nagpur City
  • Politics
  • Business
  • Nagpur Education
  • Entertainment
  • Nagpur Sports
  • Health
  • Vidarbha
  • Crime
  • Obituary
Health & Wellness

New low-cost novel coronavirus test developed

by TLN Team June 11, 2020
written by TLN Team June 11, 2020
New low-cost novel coronavirus test developed

The Indian Council of Medical Research (ICMR) has recommended only a reverse transcription-polymerase chain reaction (RT-qPCR) test for novel coronavirus testing. Researchers at the Centre for Cellular and Molecular Biology (CCMB) have developed a new low-cost and low-tech required test for SARS-CoV-2 testing. This test is known as reverse transcription nested PCR (RT-nPCR) test.

This test does not require real-time quantitative RT-qPCR. The RT-nPCR developed by the CCMB research team has shown  comparable performance to the standard RT-qPCR test. The nested PCR (RT-nPCR) approach does not depend on RT-qPCR but uses standard RT-PCR as part of an endpoint assay.

In the course of comparing the results of both tests, researchers found that the standard RT-qPCR test can have low detection efficiency (less than 50%) in a real testing scenario, which may be due to low viral representation in many samples. This finding brought home the importance of monitoring detection efficiency directly in test environments.

“We developed and tested an RT-nPCR protocol comprising a multiplex primary RT-PCR for amplification of four SARS-CoV-2 amplicons and a control human amplicon followed by a secondary nested PCR for individual amplicons. We also examined the use of RT-nPCR in pooled testing and in direct amplification without RNA isolation,” said Dr Rakesh Mishra, Director, CCMB, while speaking with India Science Wire.

The RNA isolated from nasopharyngeal swab samples that had been previously tested using one of the two RT-qPCR tests was examined using RT-nPCR and the results were compared. It was found that taking both standard RT-qPCR tests together, the RT-nPCR test was able to identify 90% of the detected samples as positive by RT-qPCR. It also detected 13% samples as positive among samples that were negative by the standard RT-qPCR test (likely false negatives). Based on the experimentally measured false negative rate by RT-nPCR tests from this study, it was estimated that as many as 50% of positive samples may escape detection in single pass testing by RT-qPCR in an actual testing scenario.

“This new test is awaiting approval from ICMR. We might ask ICMR to use this test in those places where there are no RT-qPCR machines,” remarked Dr Mishra.

0 comment
FacebookTwitterLinkedin
TLN Team

previous post
The all-new BMW X6 launched in India
next post
EPFO leverages CSC network to conduct Jeevan Pramaan for pensioners

You may also like

Hospital wise allotment of Remdesivir issued in Nagpur...

April 19, 2021

All above 18 yr eligible from May 1...

April 19, 2021

Nagpur distt reports all-time high 113 deaths, 6364...

April 19, 2021

Nagpur receive new stock of Remdesivir, Oxygen

April 19, 2021

Centre Point School organises vaccination drive by NMC

April 17, 2021

Waive GST on life-saving drugs: Pharmacists

April 17, 2021

Cause of concern, Nagpur distt reports 79 deaths,...

April 17, 2021

Hotel Center Point to facilitate as COVID Care...

April 17, 2021

Nagpur Police to conduct RAT of those roaming...

April 17, 2021

With 2,34,692 new cases India’s COVID-19 tally reaches...

April 17, 2021

Leave a Comment Cancel Reply

Save my name, email, and website in this browser for the next time I comment.

Trending News

  • April 19, 2021
  • UPSC postpones civil services examination, EPFO exam

    April 19, 2021
  • Haffkine gets permission to produce covaxin

    April 16, 2021
  • IPL 2021 to start today without spectators; opening match between RCB & MI

    April 9, 2021
  • Justice NV Ramana appointed as new Chief Justice Of India

    April 6, 2021

Important Links

  • Politics
  • Crime
  • Business
  • Education
  • Entertainment
  • Sports

Available on Playstore & Coming Soon on Appstore

Search

June 2020
M T W T F S S
« May   Jul »
1234567
891011121314
15161718192021
22232425262728
2930  

Sign-Up Form

Subscribe my Newsletter for new blog posts, tips & new photos. Let's stay updated!

Footer Logo
  • Nagpur City
  • Nagpur Education
  • Nagpur Sports
  • Business
  • Crime
  • Entertainment & Lifestyle
  • Featured
  • Health & Wellness
  • Nation
  • Politics
  • Trending
  • Vidarbha
  • Obituary
  • Privacy Policy
  • Disclaimer
  • Cookie Policy
  • Terms Of Use
  • Advertise with Us
  • Work with us
  • Contact Us

© 2020 - The Live Nagpur . All Rights Reserved.