The Live Nagpur
  • Nagpur City
  • Nagpur Education
  • Nagpur Sports
  • Business
  • Featured
  • Crime
  • Entertainment & Lifestyle
  • Health & Wellness
  • Nation
  • Politics
  • Trending
  • Vidarbha
  • Obituary

The Live Nagpur


  • Home
  • City
  • Politics
  • Business
  • Education
  • Entertainment
  • Sports
  • Health
  • Crime
  • Forest
  • Featured
  • Vidarbha
Health & Wellness

New low-cost novel coronavirus test developed

by TLN Team June 11, 2020
written by TLN Team June 11, 2020
New low-cost novel coronavirus test developed

The Indian Council of Medical Research (ICMR) has recommended only a reverse transcription-polymerase chain reaction (RT-qPCR) test for novel coronavirus testing. Researchers at the Centre for Cellular and Molecular Biology (CCMB) have developed a new low-cost and low-tech required test for SARS-CoV-2 testing. This test is known as reverse transcription nested PCR (RT-nPCR) test.

This test does not require real-time quantitative RT-qPCR. The RT-nPCR developed by the CCMB research team has shown  comparable performance to the standard RT-qPCR test. The nested PCR (RT-nPCR) approach does not depend on RT-qPCR but uses standard RT-PCR as part of an endpoint assay.

In the course of comparing the results of both tests, researchers found that the standard RT-qPCR test can have low detection efficiency (less than 50%) in a real testing scenario, which may be due to low viral representation in many samples. This finding brought home the importance of monitoring detection efficiency directly in test environments.

“We developed and tested an RT-nPCR protocol comprising a multiplex primary RT-PCR for amplification of four SARS-CoV-2 amplicons and a control human amplicon followed by a secondary nested PCR for individual amplicons. We also examined the use of RT-nPCR in pooled testing and in direct amplification without RNA isolation,” said Dr Rakesh Mishra, Director, CCMB, while speaking with India Science Wire.

The RNA isolated from nasopharyngeal swab samples that had been previously tested using one of the two RT-qPCR tests was examined using RT-nPCR and the results were compared. It was found that taking both standard RT-qPCR tests together, the RT-nPCR test was able to identify 90% of the detected samples as positive by RT-qPCR. It also detected 13% samples as positive among samples that were negative by the standard RT-qPCR test (likely false negatives). Based on the experimentally measured false negative rate by RT-nPCR tests from this study, it was estimated that as many as 50% of positive samples may escape detection in single pass testing by RT-qPCR in an actual testing scenario.

“This new test is awaiting approval from ICMR. We might ask ICMR to use this test in those places where there are no RT-qPCR machines,” remarked Dr Mishra.

0 comment
FacebookTwitterLinkedin
TLN Team

previous post
The all-new BMW X6 launched in India
next post
EPFO leverages CSC network to conduct Jeevan Pramaan for pensioners

You may also like

“Massage Therapy by Ashadeep is a step towards...

May 16, 2022

Retirement age limit of Public health docs reduced...

May 13, 2022

Diet counselling for children with special needs on...

May 12, 2022

Technology can make India self-reliant: Dr Sonde

May 12, 2022

Taking Care of Nutrition Needs of Kids with...

May 11, 2022

Knee replacement surgeries started under the MJPJAY scheme...

May 11, 2022

Vaccination: The Key to Preventing Influenza Infections amongst...

April 27, 2022

DCGI grants emergency use of Covaxin, Corbevax vaccine...

April 26, 2022

Wockhardt Hospital’s Personalized Cancer care bringing a revolutionary...

April 23, 2022

India logs 2,183 new cases, 214 deaths

April 18, 2022

Trending News

  • Mini MBA course in just one rupee, know how

    May 17, 2022
  • Know how to block spam calls on iPhone

    May 16, 2022
  • India reeling under 50°Celsius is a matter of concern

    May 16, 2022
  • Rohit Sharma, K L Rahul to be rested for T20 series

    May 15, 2022
  • Australian Cricket Star Andrew Symonds Dies In Car Crash

    May 15, 2022

Important Links

  • Politics
  • Crime
  • Business
  • Education
  • Entertainment
  • Sports

Available on Playstore & Coming Soon on Appstore

Search

June 2020
M T W T F S S
« May   Jul »
1234567
891011121314
15161718192021
22232425262728
2930  

Sign-Up Form

Subscribe my Newsletter for new blog posts, tips & new photos. Let's stay updated!

Footer Logo
  • Nagpur City
  • Nagpur Education
  • Nagpur Sports
  • Business
  • Featured
  • Crime
  • Entertainment & Lifestyle
  • Health & Wellness
  • Nation
  • Politics
  • Trending
  • Vidarbha
  • Obituary
  • Privacy Policy
  • Disclaimer
  • Cookie Policy
  • Terms Of Use
  • Advertise with Us
  • Work with us
  • Contact Us

© 2020 - The Live Nagpur . All Rights Reserved.